MarpolBase - Evolutionary Refinement of Mitochondrial and Plastid Targeting Sequences Coincides with the Late Diversification of Land Plants.

Evolutionary Refinement of Mitochondrial and Plastid Targeting Sequences Coincides with the Late Diversification of Land Plants.

Raval PK, García García C, Sanchez MS, Gould SB. · 2025 · Molecular biology and evolution research

Gene ID	Name	Evidence / Role	Function (this paper)
Mp4g02270	MpMME1 missing	experimental subject	The MpMME1 malic enzyme homolog was amplified from Marchantia cDNA, fused to a fluorescent reporter and expressed in Marchantia to test targeting. Its mitochondrial targeting peptide normally directs it to mitochondria; when the positively charged residues of the mTP were substituted with serines the construct instead predominantly targeted plastids, showing that charge divergence in the targeting sequence determines organellar specificity.
Mp2g16290	MpPAP9 missing	experimental subject	The MpPAP9 purple acid phosphatase homolog was amplified from Marchantia cDNA and its localization tested in Marchantia. It encodes a land plant-like C-terminal transmembrane domain and the fusion construct localized to plastids, supporting the paper's analysis of how targeting-sequence features dictate organellar destination.