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A tRNA-gRNA multiplexing system for CRISPR genome editing in Marchantia polymorpha.

Frangedakis E, Yelina NE, Eeda SK, Romani F, Fragkidis A, Haseloff J, Hibberd JM. · 2026 · Journal of experimental botany   research

doi:10.1093/jxb/eraf433   PMID:41025871

Linked genes (4)  0 core 4 peripheral

Gene ID	Name	Evidence / Role	Function (this paper)
—	MpGLK missing	experimental tool	Edited as a proof-of-concept target to validate the tRNA-gRNA multiplexing CRISPR system (used for genotyping/efficiency tests), not functionally characterized here.
Mp2g13020	MpRR-MYB2	experimental tool	Targeted (with MpRR-MYB5) to validate multiplexed CRISPR editing efficiency; used as a test target, not functionally studied.
Mp5g11830	MpRR-MYB5	experimental tool	Targeted to demonstrate multiplexed CRISPR editing with the tRNA-gRNA system; used as a test target.
—	MpU6 missing	experimental tool	MpU6 promoter used to drive gRNA expression in the multiplexing CRISPR constructs.