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Protocol for chromatin immunoprecipitation using isolated antheridia of Marchantia polymorpha.

Inoue K, Kotani K, Yamaoka S, Araki T. · 2026 · STAR protocols   research

doi:10.1016/j.xpro.2026.104388   PMID:41723811   PMC12938844

Linked genes (9)  3 core 6 peripheral

Gene ID	Name	Evidence / Role	Function (this paper)
Mp1g13010	MpDUO1	experimental subject	MpDUO1 is the transcription factor studied in this ChIP protocol on isolated antheridia; a Citrine knock-in line (MpDUO1-Citrineki) provides the fluorescent-tagged endogenous protein for immunoprecipitation, and a proMpDUO1:mCitrine-NLS line serves as negative control. ChIP-qPCR shows MpDUO1 enrichment near target gene TSSs bearing the DUO1-binding motif.
Mp4g08430	MpTUA5	experimental subject	Antheridium-specific tubulin gene used as a positive ChIP-qPCR target region; MpDUO1 signal is enriched near its TSS where a DUO1-binding motif lies.
Mp3g13980	MpTUB4	experimental subject	Antheridium-specific tubulin gene used as a positive ChIP-qPCR target region validating MpDUO1 binding near its TSS.
Mp8g12620	MpTUA3	experimental tool	Used as a negative-control qPCR locus because its expression is very low in antheridia and sperm.
Mp4g00550	MpTUA1	citation background	Listed as a MarpolBase accession reference.
Mp1g24530	MpTUA2	citation background	Listed as a MarpolBase accession reference.
Mp2g23500	MpTUA4	citation background	Listed as a MarpolBase accession reference.
Mp6g11660	MpTUA6	citation background	Listed as a MarpolBase accession reference.
Mp3g11780	MpTUA7	citation background	Listed as a MarpolBase accession reference.