Forestier ECF, Asprilla P, Bonter I, Romani F, Frangedakis E, Haseloff J. · 2026 · Communications biology research
doi:10.1038/s42003-025-09508-4 PMID:41772152 PMC13068942
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp8g13070 | MpABCG1 | experimental subject | Central subject of the paper. MpABCG1 is an ABCG-family transporter localized at the oil body membrane; CRISPR knockout dramatically reduced sesquiterpene accumulation in oil bodies, while overexpression mildly increased endogenous sesquiterpene levels, indicating MpABCG1 mediates sesquiterpene transport/accumulation into oil bodies. |
| Mp6g04420 | MpDXR1 | experimental subject | MEP pathway step-2 enzyme whose localization was characterized in oil body cells in this work. |
| Mp8g01240 | MpDXR2 | experimental subject | DXR isoform reported as oil body-specific in expression/localization within this study. |
| Mp2g09130 | MpDXS1 | experimental subject | MEP pathway entry enzyme studied for subcellular localization; MpDXS1 localized to plastids with enhanced expression in oil body cells. |
| Mp4g14720 | MpDXS2 | experimental subject | Second DXS isoform; localized to plastids and associated with oil body cell isoprenoid biosynthesis in this study. |
| — | MpFPS missing | experimental subject | Farnesyl pyrophosphate synthase (corresponding to IDS1) producing the sesquiterpene precursor FPP; shown to be cytosolic in oil body cells, consistent with cytosolic sesquiterpene biosynthesis. |
| Mp6g04320 | MpFTPSL2 | experimental subject | Endogenous sesquiterpene synthase (fungal terpene synthase-like 2) previously characterized; in this paper preliminary localization evidence places it partly in the oil body lumen, consistent with sesquiterpene synthesis associated with oil bodies. |
| — | MpGGPPS missing | experimental subject | Geranylgeranyl pyrophosphate synthase (re-annotated from MpIDS2) producing the diterpene precursor; characterized as plastid-localized in this study. |
| Mp5g09880 | MpHDR | experimental subject | Final MEP pathway step enzyme; localized to plastids and proposed to be under bidirectional promoter regulation with MpHDS. |
| Mp5g09870 | MpHDS | experimental subject | Late MEP pathway enzyme; localized to plastids and suggested to share bidirectional promoter regulation with MpHDR. |
| — | MpHMGR missing | experimental subject | Rate-limiting MVA pathway enzyme; localized to the ER and Golgi in oil body cells. A truncated feedback-insensitive form (Mp-tHMGR) was also tested for boosting precursor flux. |
| Mp3g01340 | MpMVD | experimental subject | Mevalonate diphosphate decarboxylase reported with oil body-specific expression/localization in this study. |
| Mp4g01910 | MpSQS3 | experimental subject | Squalene synthase reported as broadly expressed and associated with sterol biosynthesis; its localization was examined in this study. |
| Mp5g23030 | MpACT2 | sequence background | Acetoacetyl-CoA thiolase (MVA pathway) listed in the gene table with predicted peroxisomal/soluble localization; not experimentally characterized. |
| Mp3g02320 | MpC1HDZ | citation background | Cited transcription factor implicated in oil body cell differentiation from prior work. |
| Mp6g08690 | MpERF13 | citation background | Cited as a transcription factor involved in oil body cell differentiation from prior developmental work. |
| Mp4g22640 | MpHMGS | sequence background | MVA pathway enzyme listed among annotated terpene biosynthesis genes; not experimentally characterized here. |
| Mp4g00610 | MpIDI1 | sequence background | Isopentenyl diphosphate isomerase listed among annotated terpene pathway genes; not experimentally characterized. |
| Mp6g12890 | MpIDI2 | sequence background | Second isopentenyl diphosphate isomerase isoform listed in the gene tables; not experimentally characterized. |
| Mp3g22530 | MpIDS1 | sequence background | Annotation corresponding to MpFPS, the farnesyl pyrophosphate synthase characterized in this study. |
| Mp2g13280 | MpIDS2 | sequence background | Former annotation re-annotated in this paper as MpGGPPS (geranylgeranyl pyrophosphate synthase). |
| Mp5g21200 | MpIDS3 | sequence background | Alternative isoprenyl diphosphate synthase analyzed in silico; lower expression or distinct predicted function compared with the characterized IDS genes. |
| Mp5g20580 | MpIDS4 | sequence background | Alternative isoprenyl diphosphate synthase listed/analyzed with lower expression or different predicted function. |
| Mp1g06110 | MpIDS5 | sequence background | Alternative isoprenyl diphosphate synthase listed/analyzed with lower expression or different predicted function. |
| Mp4g13410 | MpMK | sequence background | Mevalonate kinase listed among MVA pathway enzymes in the gene tables; not experimentally characterized. |
| — | MpMYB2 missing | citation background | Cited transcription factor implicated in oil body cell differentiation. |
| Mp8g14400 | MpPMK | sequence background | Phosphomevalonate kinase listed among MVA pathway enzymes; not experimentally characterized. |
| Mp2g21100 | MpSQS1 | sequence background | Squalene synthase isoform listed among annotated genes; not experimentally characterized here. |
| Mp3g14160 | MpSQS2 | sequence background | Squalene synthase isoform listed among annotated genes; not experimentally characterized here. |
| Mp2g13330 | MpTGA | citation background | Cited transcription factor implicated in oil body cell differentiation. |