Higo, A., et al. · 2018 · Nature Communications research
doi:10.1038/s41467-018-07728-3 PMID:30538242 PMC6290024
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp1g00710 | MpCEN1 | experimental subject | Homolog of CENTRIN, essential for the motility apparatus; expression correlates with and depends on MpDUO1 activity. |
| Mp4g11380 | MpDAZ1 | experimental subject | Marchantia ortholog of AtDAZ1; a downstream transcription factor whose expression is controlled by MpDUO1, demonstrating conservation of the DUO1-DAZ1 module among land plants. |
| Mp1g13010 | MpDUO1 | experimental subject | MpDUO1 is the central R2R3-MYB transcription factor of this study, expressed in the sperm-cell lineage (spermatid mother cells) where its protein accumulates in the nucleus. Male Mpduo1 knockout plants are sterile, and complementation with wild-type MpDUO1 restores function. It directly controls downstream spermatogenesis genes including MpDAZ1. |
| Mp4g01100 | MpGCS1 | experimental subject | Ortholog of AtGCS1/HAP2 involved in gamete fusion; expressed independently in differentiating sperm and NOT under MpDUO1 control, showing divergence of the regulatory network. |
| Mp4g06790 | MpGEX2 | experimental subject | Ortholog of AtGEX2 involved in gamete attachment; not controlled by MpDUO1 and evolved prior to DUO1 in ancestors of plants, algae, and animals, indicating independent regulatory evolution. |
| Mp6g01560 | MpLC7 | experimental subject | Homolog of DYNEIN LIGHT CHAIN 7 (LC7); expression follows and depends on MpDUO1 as part of the sperm morphogenesis program. |
| Mp1g11830 | MpMID | experimental subject | RWP-RK transcription factor co-expressed with MpDUO1, but its loss does not affect MpDUO1 expression and male Mpmid knockout mutants remain fertile, indicating a non-essential role contrasting with its central role in Volvox. |
| — | MpPACRG | experimental subject | Ortholog of PARKIN COREGULATED (PACRG) associated with the axoneme and essential for sperm formation in mammals; its expression follows and depends on MpDUO1. |
| Mp3g14390 | MpPRM | experimental subject | PROTAMINE-LIKE gene encoding an arginine-rich nuclear protein under MpDUO1 control, illustrating rewiring of the downstream network and replacing histone variants seen in flowering plants. |
| Mp3g04030 | MpRKD | experimental subject | RWP-RK transcription factor expressed earlier than and independently of MpDUO1; plays a minor role in spermatogenesis compared with MpDUO1 and was used in complementation experiments. |
| Mp4g08430 | MpTUA5 | experimental subject | Antheridium-specific alpha-tubulin gene whose expression follows and depends on MpDUO1, identified as a sperm differentiation marker in the downstream network. |
| Mp3g13980 | MpTUB4 | experimental subject | Antheridium-specific beta-tubulin gene whose expression follows and depends on MpDUO1, linked to flagella formation during sperm differentiation. |
| Mp3g05910 | MpR2R3-MYB21 | experimental comparator | Closely related MYB transcription factor used as a negative control because it does not trans-activate HTR10; its R2R3 MYB domain served in domain-swapping experiments to define MpDUO1 binding specificity. |
| Mp6g10990 | MpACT1 | experimental tool | Actin gene used as an internal reference control for quantitative RT-PCR analysis. |