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Transcriptional and Morpho-Physiological Responses of Marchantia polymorpha upon Phosphate Starvation.

Rico-Reséndiz, F., et al. · 2020 · International Journal of Molecular Sciences   research

doi:10.3390/ijms21218354   PMID:33171770   PMC7672586

Linked genes (41)  16 core 25 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp8g09100 MpCIS1 experimental subject Citrate synthase. Up-regulated at 12 HPT, down at 24 and 150 HPT in transcriptome; qPCR showed strongly increased transcripts at 150 HPT under low Pi, supporting citrate-based organic acid response.
Mp6g07050 MpCLE1 experimental subject CLE peptide regulating meristem/thallus growth. Down-regulated at 12 and 24 HPT and confirmed by qPCR; differential expression links it to growth modulation during Pi starvation.
Mp5g18050 MpCLE2 experimental subject CLE peptide up-regulated at 12, 24, and 150 HPT under low Pi and confirmed by qPCR, implicated in growth responses to Pi starvation.
Mp1g04370 MpEDS5 experimental subject MATE citrate efflux transporter up-regulated at all time points under low Pi, consistent with citrate exudation response.
Mp7g11330 MpGARP5 experimental subject Central PHR-family MYB-CC transcription factor for internal Pi sensing. Up-regulated under low Pi at all time points and confirmed by qPCR; P1BS motif enrichment analysis was centered on its predicted targets, supporting a conserved PHR1-driven Pi starvation response.
Mp8g10550 MpLPR1 experimental subject Multicopper oxidase implicated in iron redox modification. Up-regulated at all three time points and confirmed by qPCR with high transcript levels under low Pi at 150 HPT.
Mp8g09548 MpLPR3 experimental subject Multicopper oxidase up-regulated at all three time points under low Pi alongside MpLPR1, implicated in iron redox modification during Pi starvation.
Mp1g28440 MpMATE1 experimental subject MATE citrate efflux transporter up-regulated at all time points under low Pi, suggesting preferential citrate exudation during Pi starvation.
Mp4g18470 MpMATE10 experimental subject MATE citrate efflux transporter up-regulated at all time points under low Pi, consistent with citrate exudation response.
Mp2g11400 MpMLS3 experimental subject Malate synthase in organic-acid synthesis. Down-regulated at all time points and confirmed by qPCR with significantly lower transcripts under low Pi at 12 and 150 HPT.
MpPHO1 missing experimental subject Phosphate exporter PHO1; expression promoted under low-Pi conditions in the Pi-starvation response.
Mp2g23410 MpPLD1 experimental subject Phospholipase D for phospholipid remodeling, proposed functional equivalent of AtPLDz2. Up-regulated at all three time points with the strongest fold-change among lipid-remodeling genes under Pi starvation.
Mp5g19050 MpR2R3-MYB14 experimental subject MYB transcription factor controlling auronidin biosynthesis. Transcriptional changes mainly at 150 HPT, with enriched auronidin-related GO categories linking pigment accumulation to Pi starvation.
Mp3g20340 MpRBOH2 experimental subject NADPH oxidase producing reactive oxygen species. Up-regulated at 12 and 150 HPT and down-regulated at 24 HPT, suggesting Pi limitation promotes superoxide production.
Mp1g27550 MpSPX experimental subject SPX-domain regulator of internal Pi sensing, phylogenetically closest to AtSPX4. Up-regulated across time points under low Pi and examined transcriptionally as a key component of the Pi-sensing module.
Mp8g12510 MpSTOP2 experimental subject STOP1-like transcription factor for local Pi sensing, hypothesized functionally equivalent to AtSTOP1. Down-regulated at 12 HPT and up-regulated at 24 and 150 HPT in the transcriptome, linked to organic-acid/ALMT regulation.
MpDGDG missing sequence comparator DGDG (digalactosyldiacylglycerol) biosynthesis enzyme; single homolog identified phylogenetically with conserved distribution across plant lineages.
Mp4g01560 MpGARP3 sequence comparator PHR family member identified phylogenetically, placed in a different subclade from MpPHR1 and not up-regulated under Pi stress.
Mp4g07330 MpGARP4 sequence comparator PHR family member identified phylogenetically, placed in a different subclade from MpPHR1 and not up-regulated under Pi stress.
Mp6g02120 MpMATE15 sequence comparator One of 15 MATE-type transporter loci (MpMATE1–15) identified; citrate transport candidate.
Mp2g10950 MpMGDG sequence comparator MGDG synthase in galactolipid biosynthesis; single homolog identified phylogenetically with conserved distribution across plant lineages.
Mp4g03460 MpNLA sequence comparator Ubiquitin ligase regulating Pi transporters; single homolog identified phylogenetically, close to other bryophyte sequences.
Mp3g21770 MpPAH sequence comparator Phosphatidic acid phosphohydrolase in lipid remodeling; single homolog identified phylogenetically with conserved distribution across plant lineages.
MpPHR-like missing experimental comparator PHR-like TF able to bind P1BS; Pi-starvation regulator alongside MpPHR1.
MpPHT missing experimental comparator Phosphate transporter (PHT) induced by MpPHR1 via P1BS motifs.
MpPTB missing experimental comparator PHO1-type (PTB) phosphate transporter induced via P1BS in Pi scavenging.
Mp5g24070 MpSQD sequence comparator Sulfolipid (SQDG) biosynthesis enzyme; single homolog identified phylogenetically with conserved distribution across plant lineages.
Mp2g17520 MpVPT sequence comparator Vacuolar Pi transporter; single homolog identified phylogenetically and related to other bryophyte sequences.
Mp4g14650
MpALMT2 paper
MpALMT4 nomenclature
experimental background Aluminum-activated malate transporter; down-regulated under low Pi in the transcriptome despite MpSTOP2 up-regulation.
Mp3g25480
MpALMT3 paper
MpALMT1 nomenclature
experimental background Aluminum-activated malate transporter; down-regulated under low Pi in the transcriptome.
Mp5g15980 MpCIS2 experimental background Citrate synthase family member with variable/transient expression in the transcriptome.
Mp7g15800 MpCIS5 experimental background Citrate synthase family member with variable expression in the transcriptome.
Mp8g04310 MpCIS6 experimental background Citrate synthase family member with variable expression in the transcriptome.
Mp3g04330 MpDAG experimental background Diacylglycerol-related lipid remodeling enzyme; single homolog, down-regulated at 12 and 24 HPT and up-regulated at 150 HPT in the transcriptome.
Mp8g04820 MpLPR11 experimental background Multicopper oxidase family member with variable expression in the transcriptome.
Mp3g16560 MpLPR2 experimental background Multicopper oxidase family member showing variable regulation in the transcriptome; not consistently up-regulated.
Mp8g17820 MpLPR4 experimental background Multicopper oxidase family member with variable expression in the transcriptome.
Mp8g04700 MpLPR7 experimental background Multicopper oxidase family member with variable expression in the transcriptome.
Mp6g10540
MpMLS2 paper
MpMLS1b nomenclature
experimental background Malate synthase family member with variable/transient expression in the transcriptome.
Mp8g16290 MpMLS5 experimental background Malate synthase family member with variable expression in the transcriptome.
Mp7g00270
MpNOX2 paper
MpNOX1 nomenclature
MpRBOH1 nomenclature
experimental background Second NADPH oxidase homolog; down-regulated at all three time points in the transcriptome.