Kneeshaw, S., et al. · 2022 · PNAS research
doi:10.1073/pnas.2202930119 PMID:36037336 PMC9457472
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp2g26590 | MpCOI1 | experimental subject | F-box protein component of the jasmonate coreceptor in M. polymorpha. Pull-down/binding assays showed that diverse ligands, including Δ4-dn-OPDA, facilitate the MpCOI1–MpJAZ interaction. Mpcoi1 mutants were tested in growth-inhibition assays to probe ligand activity. |
| Mp3g13250 | MpDES5 | experimental subject | Desaturase converting eicosatetraenoic acid (ETA) into EPA. Mpdes5 mutants were analyzed for EPA/ETA production and downstream jasmonate biosynthesis. |
| Mp6g17330 | MpDES6 | experimental subject | Δ6-desaturase in the EPA biosynthetic route. CRISPR Mpdes6 mutants (including double mutants) were analyzed for C20-OPDA and Δ4-dn-OPDA accumulation after wounding. |
| Mp3g10660 | MpFAD5 | experimental subject | Fatty acid desaturase in jasmonate biosynthesis. CRISPR-generated Mpfad5 mutants were analyzed for PUFA and jasmonate content and challenged in herbivory and fungal assays to assess pathway contribution. |
| Mp6g06230 | MpJAZ | experimental subject | Jasmonate-ZIM-domain protein forming the MpCOI1–MpJAZ coreceptor complex. Recombinant MpJAZ was used in pull-down assays to assess ligand-dependent coreceptor interaction. |
| Mp7g06530 | missing | experimental tool | Aromatic L-amino acid/L-tryptophan decarboxylase gene tested by qPCR as a wound-responsive marker. |
| Mp3g25140 | MpAPT | experimental tool | Reference gene (Mp3g25140) used for qPCR normalization of gene expression. |
| Mp2g10330 | MpCYP94-like | experimental tool | Cytochrome P450 CYP94-like gene (Mp2g10330) measured by qPCR as a wound-responsive expression marker. |
| Mp3g23400 | MpEF1 | experimental tool | Reference gene used to normalize DNA levels in qPCR-based fungal quantification. |
| Mp5g21680 | MpLOX4 | experimental tool | Wound-responsive lipoxygenase gene (Mp5g21680) used as a marker in qPCR expression analyses. |
| Mp5g21760 | MpPATATIN missing | experimental tool | Patatin-like phospholipase gene measured by qPCR as a wound-responsive marker. |
| Mp6g02350 | MpELO1 | citation background | Δ6-elongase mentioned only as part of the described ALA-to-EPA biosynthetic pathway. |