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Circadian clock does not play an essential role in daylength measurement for growth-phase transition in Marchantia polymorpha.

Kanesaka, Y., et al. · 2023 · Frontiers in Plant Science   research

doi:10.3389/fpls.2023.1275503   PMID:38023914   PMC10673691

Linked genes (10)  2 core 8 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp2g16560 MpPRR experimental subject CRISPR/Cas9 knockout mutant generated; its promoter also drove a luciferase reporter for monitoring clock rhythms. Mutation caused no significant change in gametangiophore formation timing under tested daylengths.
Mp3g09460 MpTOC1 experimental subject CRISPR/Cas9 knockout mutant generated in a luciferase-reporter background. Loss of this clock gene had no significant effect on the timing of gametangiophore formation, supporting that the clock is dispensable for daylength measurement.
Mp1g18540 MpCDF sequence comparator Noted as the single-copy M. polymorpha ortholog/counterpart of Arabidopsis CDF in a sequence comparison.
Mp6g17600 MpPAPK1.2 experimental tool Single-copy CK1 gene (Mp6g17600) targeted by the casein kinase 1 inhibitor AMI-331, which lengthens the circadian period in M. polymorpha. Used pharmacologically to perturb the clock and test its role in daylength measurement.
Mp3g14360 MpFKF citation background Cited from prior work: knockout suppressed and overexpression promoted the growth-phase transition irrespective of daylength.
Mp1g13750 MpGI citation background Cited from prior work showing a diel expression pattern like its Arabidopsis orthologue, implicating it in daylength response.
Mp5g20660 MpGRAS6 citation background Cited as a possible downstream factor acting after daylength measurement.
MpKANADI missing citation background Cited as a possible downstream factor acting after daylength measurement.
Mp3g17350 MpPIF citation background Cited as a possible downstream factor acting after daylength measurement.
Mp1g10030 MpSPL2 citation background Cited as part of the miR156/529-SPL module; miR529c restricts MpSPL2 activity to block reproductive transition under SD.