Wang, M., et al. · 2024a · Communications Biology research
doi:10.1038/s42003-023-05746-6 PMID:38267515 PMC10808223
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp8g16970 | MpPGDH missing | experimental subject | Main study gene; 3-phosphoglycerate dehydrogenase of the phosphorylated serine biosynthesis pathway. CRISPR/Cas9 knockouts show serine supply in the dark is crucial for thallus growth and that MpPGDH-mediated serine synthesis is essential for sperm, embryo, and sporophyte development. Recombinant protein (65.6 kDa) shows AtPGDH1-like cooperative inhibition by L-serine. |
| Mp1g15430 | MpPSAT missing | experimental subject | 3-phosphoserine aminotransferase, second enzyme of the phosphorylated pathway. Its expression together with MpPGDH was significantly induced 8 and 16 h after transfer to darkness, supporting activation of the pathway under dark conditions. |
| Mp6g10990 | MpACT1 | experimental tool | Reference gene used to normalize relative expression levels in qRT-PCR. |
| Mp1g13010 | MpDUO1 | experimental tool | Sperm differentiation marker (upstream regulator of MpPRM); its expression was similar in wild type and Mppgdh mutants in the qRT-PCR panel, distinguishing affected from unaffected steps. |
| Mp3g14390 | MpPRM | experimental tool | Late-spermiogenesis sperm-specific marker; significantly repressed in Mppgdh mutants in the qRT-PCR sperm-differentiation panel, used as a readout of impaired sperm development. |
| Mp1g00710 | MpCEN1 | sequence background | Listed only in the data availability/accession section (Mp1g00710); no functional analysis. |
| Mp4g11380 | MpDAZ1 | sequence background | Listed only in the data availability/accession section (Mp4g11380); no functional analysis. |
| Mp2g07580 | MpGDH missing | experimental background | Glycerate dehydrogenase of the glycerate pathway; qRT-PCR shows gradual downregulation in darkness as an alternative serine route. |
| Mp6g01560 | MpLC7 | sequence background | Listed only in the data availability/accession section (Mp6g01560); no functional analysis. |
| Mp1g11830 | MpMID | sequence background | Listed only in the data availability/accession section (Mp1g11830); no functional analysis. |
| Mp2g10500 | MpPSP missing | sequence background | 3-phosphoserine phosphatase, named as the third enzyme of the phosphorylated pathway; no experimental functional data presented. |
| Mp3g04030 | MpRKD | sequence background | Listed only in the data availability/accession section (Mp3g04030); no functional analysis. |
| Mp1g09830 | MpSHMT missing | experimental background | Serine hydroxymethyltransferase of the glycolate pathway; measured by qRT-PCR and gradually reduced in darkness, cited as an alternative serine route. |
| Mp4g08430 | MpTUA5 | sequence background | Listed only in the data availability/accession section (Mp4g08430); no functional analysis. |