Das, S., et al. · 2024 · Plant Communications research
doi:10.1016/j.xplc.2024.101039 PMID:38988072 PMC11589480
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp1g12750 | MpARF1 | experimental subject | Class A activator ARF; MpARF1-mScarlet-I showed the highest accumulation among NAP components across most cell types during gemma germination. Its protein levels progressively declined and it was shown to be proteasomally degraded, tuning ARF stoichiometry for auxin-responsive gene activation. |
| Mp4g11820 | MpARF2 | experimental subject | Repressor-class ARF; MpARF2-mScarlet-I accumulated weakly and was absent distal to the apical notch. Like MpARF1, it is proteasomally degraded and its levels decline during germination, contributing to regulated ARF stoichiometry. |
| Mp1g07070 | MpARF3 | experimental subject | Class C ARF; MpARF3-mScarlet-I displayed a weak but broad accumulation in all cell types. Unlike MpARF1/MpARF2, it was unaffected by proteasome-inhibitor treatment and its pattern did not change, serving as a non-degraded contrast. |
| Mp6g05000 | MpIAA | experimental subject | Sole canonical Aux/IAA repressor; undetectable in dormant gemma, MpIAA-mScarlet-I underwent rapid auxin-triggered degradation imaged in vivo, with a measured half-life of ~6.5 min, reporting on TIR1-mediated auxin signaling dynamics. |
| Mp2g02890 | MpncARF | experimental subject | Non-canonical ARF lacking the DNA-binding domain; MpncARF-mScarlet-I showed low nuclear accumulation and positively influences auxin response in this system. |
| Mp6g02750 | MpTIR1 | experimental subject | Sole TIR1 auxin receptor; MpTIR1-mScarlet-I showed low nuclear accumulation, was detectable at all stages with a gradual increase, and was shown active throughout gemma development in mediating IAA-triggered MpIAA degradation. |
| Mp3g23400 | MpEF1 | experimental tool | Housekeeping gene used as a reference for transcript-level normalization. |
| Mp4g07110 | MpSAND missing | experimental tool | Housekeeping gene used as a reference for transcript-level normalization. |
| Mp8g07850 | MpncIAA | citation background | Non-canonical Aux/IAA mentioned in passing as a component of the minimal Marchantia auxin module. |