Hernández-García, J., et al. · 2024a · Nature Communications research
doi:10.1038/s41467-024-55278-8 PMID:39738167 PMC11685440
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp1g12750 | MpARF1 | experimental subject | An A-class ARF studied as the central genetic model for functional conservation. Knock-out Mparf1 mutants were complemented with wild-type and chimeric constructs, and domain-swap, PB1-interaction, and complementation assays defined A-class-specific activity. |
| Mp4g11820 | MpARF2 | experimental subject | A B-class repressor ARF analyzed for class-specific function. Tested for complementation capacity in Mparf1 mutants (failed like other non-A classes), and PB1-interaction assays showed strong heterotypic interaction between MpARF2 and MpARF3 PB1 domains. |
| Mp1g07070 | MpARF3 | experimental subject | A C-class ARF used as a model for C-class function. Mparf3 mutants underwent complementation and domain-swap assays for apical growth and gemma formation; non-C classes could not complement, demonstrating C-class specificity. |
| Mp2g00710 | MpBromo4 | experimental subject | A pre-ARF-related gene investigated as the evolutionary precursor lineage of ARFs. CRISPR/Cas9 Mpphip mutants were generated and showed no genetic or functional resemblance to MpARF1 or MpARF3, and structural alignment of its DD-AD region was compared with ARF. |
| Mp2g22250 | MpRAV | experimental subject | Studied as a domain-sharing relative used to probe ARF domain function and evolutionary divergence. MpRAV B3 and PB1 domains were swapped into MpARF1/MpARF3 chimeras for complementation tests, and yeast two-hybrid assays showed MpRAV PB1 strongly self-interacted. |
| Mp3g23400 | MpEF1 | experimental tool | Used as a reference (housekeeping) gene; co-used with MpSAND for qRT-PCR expression normalization. |
| Mp6g05000 | MpIAA | experimental tool | Aux/IAA repressor used to probe PB1-domain function and auxin regulation; its PB1 domain in MpARF1 chimeras partly restored growth and auxin responsiveness, and it served in yeast two-hybrid interaction profiling. |
| Mp4g07110 | MpSAND missing | experimental tool | Used as a reference (housekeeping) gene; its expression contributed to the geometric mean for qRT-PCR normalization. |
| Mp1g09500 | MpWIP | experimental tool | Used as an auxin-responsive marker to validate auxin signaling; its expression was monitored to confirm normal auxin response in mutant plants. |
| — | MpWIP1 missing | experimental tool | Auxin-responsive marker gene measured by qRT-PCR to confirm normal auxin-dependent gene expression in MpphipGE mutants. |