MarpolBase - Evolutionary-conserved RLF, a cytochrome b5-like heme-binding protein, regulates organ development in Marchantia polymorpha.

Evolutionary-conserved RLF, a cytochrome b5-like heme-binding protein, regulates organ development in Marchantia polymorpha.

Iwata KP, Shimizu T, Sakai Y, Furuya T, Fukumura H, Kondo Y, Masuda T, Ishizaki K, Fukaki H. · 2025 · The New phytologist research

Gene ID	Name	Evidence / Role	Function (this paper)
Mp3g04390	MpRLF missing	experimental subject	MpRLF, a cytochrome b5-like heme-binding protein, is the focus of this paper. CRISPR/Cas9 loss-of-function mutants (Mprlf-ge) showed delayed thallus growth and impaired gemma cup and reproductive organ formation; estradiol-inducible MpE2Fpro:XVE>>MpRLF overexpression and MpRLFpro:GUS reporter analyses were performed. MpRLF binds heme in vitro and rescues the Arabidopsis rlf mutant, showing functional conservation.
Mp1g02890	MpE2F	experimental tool	The MpE2F promoter (MpE2Fpro) was used as a driver in XVE estradiol-inducible constructs to conditionally express MpRLF.
Mp6g04830	MpGCAM1	experimental background	GCAM1/MpGCAM1 expression was decreased in Mprlf-ge mutants in RNA-seq.
Mp5g06550	MpKARAPPO	experimental background	KARAPPO expression was unchanged in Mprlf-ge mutants in RNA-seq.
Mp3g04030	MpRKD	experimental background	MpRKD expression was decreased in Mprlf-ge mutants in RNA-seq.