Linde, A.-M., et al. · 2017 · New Phytologist research
doi:10.1111/nph.14487 PMID:28244104 PMC5638080
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| — | MpEFL missing | experimental subject | Single ELF4-like homologue that did not show rhythmic expression under any tested condition; not analyzed further by mutant or reporter assays. |
| Mp1g10870 | MpELF3 | experimental subject | Single ELF3 homologue with evening-phased circadian expression; promoter:LUC shows rhythmic expression in neutral day and constant light, localized to meristematic regions. |
| Mp3g14360 | MpFKF | experimental subject | Sole ZTL/FKF1-family member; did not show significant circadian rhythmicity. Interacts with MpGI in the photoperiodic pathway. |
| Mp1g13750 | MpGI | experimental subject | Single GIGANTEA gene showing circadian rhythmicity; interacts with the sole ZTL-family member MpFKF and affects photoperiodic reproduction. Promoter:LUC rhythmic in meristematic regions. |
| Mp4g21220 | MpLUX | experimental subject | Single LUX ARRHYTHMO homologue with evening-phased circadian expression; promoter:LUC shows bioluminescence rhythms under day/night and constant light, expressed in meristematic regions. |
| Mp2g16560 | MpPRR | experimental subject | PRR-family gene clustering with the AtPRR3/7 clade; shows the strongest circadian transcriptional oscillation under day/night and constant light. Negatively regulated by MpTOC1 and positively by MpRVE; knockout disrupts bioluminescence patterning. Strongly expressed in meristematic and apical regions. |
| Mp2g14310 | MpRVE | experimental subject | Single REVEILLE homologue clustering with the LCL clade; shows diel/circadian rhythmic expression in mature thalli. Knockout abolished 35S:LUC bioluminescence rhythm, and MpRVE positively regulates MpPRR. Promoter:LUC expression localized to meristematic and developing tissues. |
| Mp3g09460 | MpTOC1 | experimental subject | Single TOC1 homologue clustering with the AtTOC1 clade; shows diel/circadian expression. Knockout abolished rhythmic bioluminescence under constant light, and MpTOC1 directly or indirectly represses MpPRR. |
| — | MpACT missing | experimental tool | Used as a reference/control gene for qRT-PCR expression normalization. |
| Mp3g23400 | MpEF1 | experimental tool | ELONGATION FACTOR 1α used as a reference/control gene for qRT-PCR expression normalization. |
| Mp2g09390 | MpTUB2 | experimental tool | MpβTubulin2 used as a reference/control gene for qRT-PCR expression normalization. |