Lagerkrantz, U., et al. · 2020 · Scientific Reports research
doi:10.1038/s41598-020-65372-8 PMID:32457350 PMC7251115
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp1g12750 | MpARF1 | experimental subject | microRNA knockdown line (amiR-MpARF1) reduces auxin sensitivity and attenuates the amplitude of rhythmic waving without altering its period. |
| Mp4g21220 | MpLUX | experimental subject | Two independent mutant alleles (Mpluxge-9, Mpluxge-19) show arrhythmic phenotypes with dampened rhythm and elevated MpPRR; free IAA oscillations are lost in the mutant. |
| Mp2g16560 | MpPRR | experimental subject | Knockout mutant (Mpprrko) loses rhythmic thallus movement in continuous light and shows arrhythmic clock gene expression. Forms part of a core transcriptional feedback loop; repressed by MpTOC1 and activated by MpRVE. |
| Mp2g14310 | MpRVE | experimental subject | Knockout mutant (Mprveko) shows an arrhythmic phenotype. Acts as a transcriptional activator of MpPRR and other clock genes within the core feedback loop. |
| Mp5g14320 | MpTAA | experimental subject | Encodes the first-step auxin biosynthesis enzyme; qRT-PCR shows a circadian expression pattern in wild-type that is disrupted in clock mutants, linking the clock to auxin output. |
| Mp3g09460 | MpTOC1 | experimental subject | Knockout mutant (Mptoc1ko) loses rhythmic movement in continuous light. Acts as a repressor of MpPRR and is itself repressed by MpRVE, forming the core feedback loop. |
| Mp8g08780 | MpYUC2 | experimental subject | Auxin biosynthesis enzyme; qRT-PCR detected no rhythmic expression in constant light, while overexpression produces an epinastic growth phenotype. |
| Mp3g19510 | MpSHI | experimental tool | Apical-notch-active promoter used to drive iaaL expression (MpSHIpro:iaaL) to deplete auxin locally. |
| Mp1g10870 | MpELF3 | sequence background | Reported as a homolog present in the M. polymorpha genome; not experimentally characterized here. |
| Mp1g16510 | MpELF4 | sequence background | Reported as a homolog present in the M. polymorpha genome; not experimentally characterized here. |
| Mp1g13750 | MpGI | sequence background | Reported as a homolog in the M. polymorpha genome, noted as a large protein of unclear biochemical function; not experimentally characterized here. |
| — | MpZTL missing | sequence background | Reported as a homolog present in the M. polymorpha genome; not experimentally characterized here. |