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Functions of COP1/SPA E3 Ubiquitin Ligase Mediated by MpCRY in the Liverwort Marchantia Polymorpha under Blue Light.

Zhang, L., et al. · 2022b · International Journal of Molecular Sciences   research

doi:10.3390/ijms23010158   PMID:35008588   PMC8745113

Linked genes (8)  4 core 4 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp5g12010 MpCOP1 experimental subject E3 ubiquitin ligase with RING-finger, coiled-coil, and WD40 domains; interacts with full-length MpSPA. It ubiquitinates MpHY5, a reaction promoted by MpSPA; CRISPR knockout lines were lethal, limiting full characterization.
Mp2g17590 MpCRY experimental subject Central blue-light receptor of the study; knockout lines Mpcry-7 and Mpcry-8 were generated by CRISPR/Cas9. MpCRY is degraded and phosphorylated under blue light like Arabidopsis CRY2, and constitutively interacts with MpSPA in yeast.
Mp1g16800
MpHY5 paper
MpBZIP1 nomenclature
experimental subject Ortholog of AtHY5 (Mp1g16800) and target of the COP1/SPA complex; its C-terminus is bound by the MpSPA coiled-coil domain. MpHY5 is ubiquitinated and destabilized via MpCOP1/MpSPA, but does not regulate symmetric thallus growth.
Mp3g25460 MpSPA1 experimental subject COP1-interacting regulator with kinase-like, coiled-coil, and WD40 domains; knockout lines Mpspa-8 and Mpspa-11 were generated. MpSPA accumulates under blue light and promotes MpCOP1-mediated ubiquitination of MpHY5.
Mp3g14360 MpFKF citation background ZEITLUPE-family blue-light receptor cited as background in the M. polymorpha photoreceptor repertoire.
Mp5g03810 MpPHOT citation background Phototropin blue-light receptor cited among previously known M. polymorpha photoreceptors.
Mp2g16090 MpPHY citation background Single-copy phytochrome ortholog cited as previously characterized photoreceptor context in M. polymorpha.
Mp2g11590 MpUVR8 citation background UV-B photoreceptor cited as part of the M. polymorpha light-signaling background.