MarpolBase - Diversification of heat shock transcription factors expanded thermal stress responses during early plant evolution.

Diversification of heat shock transcription factors expanded thermal stress responses during early plant evolution.

Wu, T.-Y., et al. · 2022a · Plant Cell research

Gene ID	Name	Evidence / Role	Function (this paper)
Mp4g12230	MpHSF1	experimental subject	Single-copy HSFB1 of Marchantia; Mphsfb1 CRISPR knockout studied under HS and non-HS conditions. Regulates development (meristem branching, antheridiophore formation, cell division, cell-wall, transporters) with mutants failing to form antheridiophores; induces GABA accumulation under heat, representing the rapidly evolved HSFB subclass.
MpUg00290	MpHSF2 missing	experimental subject	Female (U chromosome) variant of MpHSFA1; Mphsfa1U CRISPR knockout characterized under heat. Expressed ~2-2.5-fold lower than MpHSFA1V and its promoter lacks heat shock elements, correlating with higher heat sensitivity in female (BC3) plants.
—	MpHSFA1 missing	experimental subject	Single-copy HSFA1 gene of Marchantia, studied via CRISPR knockout, RNA-seq, metabolomics and ChIP-seq under heat stress. Controls canonical heat responses including thermotolerance and metabolic changes (proline, amino acids), regulating protein folding, chaperone and hormone-signaling genes; mutants show growth retardation, thallus senescence and increased thermal sensitivity.
MpVg00470	MpHSFA1V paper MpHSF3 nomenclature	experimental subject	Male (V chromosome) variant of MpHSFA1; Mphsfa1V knockout and overexpression/ChIP-seq lines analyzed under heat. Promoter contains 4 HSEs and is expressed higher than MpHSFA1U; direct binding targets identified, mediating thermotolerance and metabolic adjustment but not GABA accumulation.