Sakai Y, Takami H, Yamaoka S, Kato H, Fukaki H, Kohchi T, Ishizaki K. · 2025 · The New phytologist research
doi:10.1111/nph.70337 PMID:40734229 PMC12371152
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp8g11870 | MpSTG | experimental subject | Main subject of the paper; SHOT GLASS (MpSTG / MpR2R3-MYB5, Mp8g11870) is an R2R3-MYB transcription factor. Knockout (Mpstg ko/ge) plants failed to form normal gemma cups and showed impaired air chamber and gametangiophore development; complementation (gMpSTG-Citrine) rescued the phenotype and DEX-induced overexpression (MpSTG-GR) suppressed thallus growth, demonstrating MpSTG promotes gemma cup and gametangiophore development. |
| Mp6g16800 | MpWRKY14 | experimental subject | In transcriptome analysis of the inducible MpSTG-GR line, MpWRKY14 was the most strongly upregulated gene after 2-h DEX treatment, identifying it as an early MpSTG-responsive target. |
| — | MpACT8 missing | experimental tool | Used as a qPCR internal reference control. |
| Mp6g11010 | MpACTIN7 | experimental tool | Used as a qPCR internal reference control. |
| Mp3g23400 | MpEF1 | experimental tool | Used as a qPCR internal reference control. |
| Mp2g09680 | MpHA9 | experimental tool | Used as a proMpHA9:Citrine-GUS promoter reporter marking gemma cup floor cell identity to assess gemma cup development. |
| Mp3g17930 | MpRSL1 | experimental tool | Used as a proMpRSL1:tdTomato-NLS promoter reporter marking developing gemmae. |
| Mp4g21300 | Mp1R-MYB17 | experimental background | Downregulated DEG in MpSTG-induction transcriptome analysis. |
| Mp3g23300 | MpBNB | citation background | Cited bHLH (BONOBO) transcription factor in reproductive development. |
| Mp5g10030 | MpCYCB_1 | experimental background | Significantly downregulated upon MpSTG induction, consistent with reduced cell proliferation. |
| Mp4g08700 | MpGARP2 | experimental background | Downregulated DEG in MpSTG-induction transcriptome analysis. |
| Mp6g04830 | MpGCAM1 | citation background | Cited R2R3-MYB regulator of gemma cup initiation. |
| Mp5g20660 | MpGRAS6 | experimental background | Upregulated DEG in MpSTG-induction transcriptome analysis; also noted as inhibiting gametangia formation via interaction with MpPIF. |
| Mp3g04970 | MpKAN | citation background | Cited GARP transcription factor in gemma cup development context. |
| Mp1g00270 | MpLOG | citation background | Cited cytokinin biosynthesis enzyme acting upstream of MpGCAM1. |
| — | MpMIR319 missing | citation background | Cited as source of MIR319-derived miRNAs. |
| Mp3g17350 | MpPIF | citation background | Cited phytochrome-interacting factor in sexual reproduction regulation. |
| Mp3g04030 | MpRKD | citation background | Cited RWP-RK domain transcription factor regulating gemma cup rim outgrowth. |
| Mp4g20600 | MpRRB | citation background | Cited as a type-B response regulator that positively regulates MpGCAM1. |
| Mp2g25660 | MpTRIHELIX12 | experimental background | Downregulated DEG in MpSTG-induction transcriptome analysis. |
| Mp1g24950 | MpWRKY11 | experimental background | Upregulated DEG in MpSTG-induction transcriptome analysis. |