Westermann, J., et al. · 2020 · Frontiers in Plant Science research
doi:10.3389/fpls.2020.569194 PMID:33178238 PMC7593560
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp8g00450 | MpARA6 | experimental tool | Endosomal compartment marker visualized in punctate structures and co-localized with MpRAB5 as a validation pair. |
| — | MpDCP1 missing | experimental tool | P-body marker; localized in dot-like structures like AtDCP1. Used as BiFC interaction partner with MpDCP2 to validate the assay. |
| Mp8g16420 | MpDCP2 missing | experimental tool | Nucleocytoplasmic marker mirroring AtDCP2. Serves as BiFC partner co-expressed with MpDCP1 to demonstrate protein-protein interaction detection. |
| Mp3g23400 | MpEF1 | sequence tool | proMpEF1a strong promoter offered as an expression driver alongside pro35S and proAtUBQ10 for toolkit constructs. |
| Mp4g15890 | MpFER paper
MpFERONIA/MpTHESEUS nomenclature |
experimental tool | Plasma membrane marker tested with single and triple fluorescent tags to compare tagging strategies for localization studies. |
| Mp1g06880 | MpLIP5 missing | experimental tool | Negative control in BiFC; its co-expression yields no YFP signal, confirming assay specificity. |
| Mp7g17560 | MpMRI | experimental tool | Plasma membrane marker (with cytoplasmic traces) used to verify localization and triple-tag co-localization in the toolkit. |
| Mp1g08940 | MpRAB5 | experimental tool | Endosomal marker shown in punctate structures, co-localizing with MpARA6 to validate dual-marker imaging. |
| Mp8g01610 | MpSKD1 missing | experimental tool | BiFC control partner fused to YFPN, producing clear punctate YFP signal to validate the interaction assay. |
| Mp7g13190 | MpSYP13A | experimental tool | Plasma membrane marker co-transformed with AtNPSN12 to confirm expected localization for toolkit quality control. |