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KANADI promotes thallus differentiation and FR-induced gametangiophore formation in the liverwort Marchantia.

Briginshaw, L. N., et al. · 2022 · New Phytologist   research

doi:10.1111/nph.18046   PMID:35181887   PMC9311212

Linked genes (40)  3 core 37 peripheral

Gene ID Name Evidence / Role Function (this paper)
Mp5g20660 MpGRAS6 experimental subject MpDELLA. Under FR, MpKAN negatively regulates MpDELLA expression, and the two are proposed to act in a common pathway controlling FR responses and gametangiophore formation.
Mp3g04970 MpKAN experimental subject MpKAN locus (v6.1 Mp3g04970.1), the KANADI gene studied here. Loss-of-function, complementation and inducible-overexpression lines showed it regulates apical branching, dorsal thallus differentiation and FR-induced gametangiophore formation in the gametophyte.
Mp5g14320 MpTAA experimental subject MpTAA, the sole TAA auxin-biosynthesis ortholog. Transcriptomics showed it is upregulated in Mpkan and downregulated upon MpKAN induction; MpKAN-mediated thallus differentiation is proposed to act by downregulating MpTAA and depleting auxin.
Mp2g16090 MpPHY experimental comparator Phytochrome; Mpphy mutants grow orthotropically under WL and FR, used as comparative mutant context for FR-regulated growth and positive regulation of MpPIF.
Mp3g17350 MpPIF citation comparator PHYTOCHROME-INTERACTING FACTOR that interacts with MpDELLA in the FR pathway regulating gametangium/gametangiophore formation; cited as pathway context.
Mp3g23400 MpEF1 experimental tool Promoter (proMpEF1) used to drive the inducible MpKAN-GR transgene for MpKAN overexpression/induction experiments.
Mp1g28830 missing experimental background Unannotated gene; listed in Table 1 among DEGs oppositely regulated by loss and induction of MpKAN.
Mp2g02350 missing experimental background Protein of unknown function (DUF3455); listed in Table 1 as a candidate MpKAN-regulated DEG.
Mp2g13230 missing experimental background Unannotated gene; listed in Table 1 among DEGs oppositely regulated by MpKAN loss and induction.
Mp3g17960 missing experimental background Unannotated gene; listed in Table 1 as a candidate MpKAN-regulated DEG.
Mp3g21740 missing experimental background Peroxidase/oxygenase; listed in Table 1 among MpKAN-responsive DEGs.
Mp4g03600 missing experimental background Sulphite exporter (TauE/SafE); listed in Table 1 as a candidate MpKAN-regulated DEG.
Mp4g15440 missing experimental background Indole-3-acetate O-methyltransferase; listed in Table 1 as a candidate MpKAN-regulated DEG.
Mp4g16270 missing experimental background Unannotated gene; listed in Table 1 among MpKAN-responsive DEGs.
Mp5g00560 missing experimental background Methyltransferase; listed in Table 1 as a candidate MpKAN-regulated DEG.
Mp5g14990 missing experimental background Unannotated gene; listed in Table 1 as a candidate MpKAN-regulated DEG.
Mp5g16240 missing experimental background EamA-like transporter; listed in Table 1 among MpKAN-responsive DEGs.
Mp7g01790 missing experimental background 6-methylsalicylate decarboxylase; listed in Table 1 as a candidate MpKAN-regulated DEG.
Mp7g08280 missing experimental background Unannotated gene; listed in Table 1 among MpKAN-responsive DEGs.
Mp7g09830 missing experimental background Alpha/beta hydrolase; listed in Table 1 as a candidate MpKAN-regulated DEG.
Mp7g11540 missing experimental background 3-phosphoglycerate kinase; listed in Table 1 among MpKAN-responsive DEGs.
Mp7g14650 missing experimental background 3-methylcrotonyl-CoA carboxylase; listed in Table 1 as a candidate MpKAN-regulated DEG.
Mp7g16380 missing experimental background Ribonuclease III (rnc/DROSHA/RNT1); listed in Table 1 among MpKAN-responsive DEGs.
Mp8g18310 MpBELL1 experimental background TALE-homeodomain TF; FR-upregulated in wild-type and still FR-upregulated in Mpkan, indicating MpKAN-independent induction in the transcriptome analysis.
Mp3g23300 MpBNB experimental background MpBONOBO TF for gametangium specification; FR-upregulated in wild-type but not FR-upregulated in Mpkan plants, noted in the FR transcriptome comparison.
Mp2g03280 MpCCD7 missing experimental background MpCCD7 (9-cis-beta-carotene cleaving dioxygenase); listed in Table 1 as a candidate MpKAN-regulated DEG.
Mp2g03050 MpCHK1 experimental background MpCHK1 (cytokinin receptor); listed in Table 1 among genes upregulated in Mpkan and downregulated upon MpKAN induction.
Mp6g07050 MpCLE1 experimental background Meristem-associated gene; downregulated together with MpTAA in DEX-treated proMpEF1:MpKAN-GR plants in the transcriptome analysis.
Mp1g04680 MpCYP716 experimental background Cytochrome P450; listed in Table 1 as upregulated in Mpkan and downregulated in DEX-treated proMpEF1:MpKAN-GR, a candidate MpKAN-regulated DEG.
Mp8g06340 MpDIR6 experimental background Dirigent-like protein; listed in Table 1 as a candidate MpKAN-regulated DEG.
Mp1g17210 MpFGMYB experimental background Regulator of female sexual differentiation; FR-upregulated in wild-type females in the expression analysis.
Mp8g02960 MpIPT2 experimental background Cytokinin biosynthesis gene; FR-upregulated in wild-type males and females in the transcriptome analysis.
Mp1g00270 MpLOG experimental background Cytokinin biosynthesis gene; FR-upregulated in wild-type males and females in the transcriptome analysis.
Mp1g21930 MpLOX5 experimental background MpLOX5 (LOX2S lipoxygenase); appears in the Table 1 set of genes oppositely regulated between Mpkan and MpKAN-GR plants.
Mp4g12310 MpLRR-RLK.59 experimental background Serine/threonine protein kinase; listed in Table 1 among MpKAN-responsive DEGs.
Mp5g06850 MpOMT19 experimental background O-methyltransferase; listed in Table 1 among MpKAN-responsive DEGs.
Mp8g11680 MpPKC11 experimental background Polyketide cyclase/dehydrase; listed in Table 1 among MpKAN-responsive DEGs.
Mp3g15790 MpPOD28 experimental background Peroxidase; listed in Table 1 among genes oppositely regulated between Mpkan and MpKAN-GR plants.
Mp5g19050 MpR2R3-MYB14 experimental background R2R3-MYB master regulator of flavonoid biosynthesis; listed among DEGs upregulated in the Mpkan background.
Mp3g10530 MpRLK-Pelle_RKF3c experimental background Serine/threonine protein kinase; listed in Table 1 as a candidate MpKAN-regulated DEG.