Koebke, E., et al. · 2022 · Frontiers in Plant Science research
doi:10.3389/fpls.2022.915268 PMID:36212282 PMC9537460
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp2g15800 | MpSPI missing | experimental subject | Alias/short form of the study's main gene; phylogenetically the closest homolog to AtSPI by BLAST. |
| Mp2g15880 | MpSPIRRIG | experimental subject | Central subject of the study: a BEACH-domain gene characterized via a T-DNA mutant (Mpspi) in Marchantia polymorpha. |
| — | MpDCP1 missing | experimental comparator | Tested for interaction with MpSPI, but no interaction was found in pairwise yeast two-hybrid and BiFC assays. |
| Mp1g06880 | MpLIP5 missing | experimental comparator | Tested as a co-localization and interaction partner of MpSPI PBW; yeast two-hybrid showed a strong interaction that could not be independently confirmed. |
| Mp8g01610 | MpSKD1 missing | experimental comparator | Tested as a co-localization partner: MpSPI PBW co-localizes entirely with MpSKD1, but no yeast two-hybrid interaction was detectable. |
| Mp6g11010 | MpACTIN7 | citation tool | Actin reference gene used for qPCR normalization; primers described previously. |
| — | MpAPT3 missing | citation tool | Reference gene used for RT-qPCR normalization; primers described previously. |
| Mp8g00450 | MpARA6 | experimental tool | Endosomal marker (RABF1) used to test specificity; partial overlap with MpSPI supported direct MpLIP5/MpSKD1 interaction. |
| Mp8g16420 | MpDCP2 missing | experimental tool | P-body marker co-expressed with MpSPI PBW, showing strong co-localization in punctate structures. |
| Mp1g08940 | MpRAB5 | experimental tool | Endosomal marker used to test localization specificity; showed only partial overlap with MpSPI. |