Kanazawa, T., et al. · 2020 · Nature Communications research
doi:10.1038/s41467-020-19978-1 PMID:33262353 PMC7708844
| Gene ID | Name | Evidence / Role | Function (this paper) |
|---|---|---|---|
| Mp8g13070 | MpABCG1 | experimental subject | MpABCG1 locus; ABCG transporter downstream of MpERF13, targeted to oil body membrane. |
| Mp2g03080 | MpCLC1 | experimental subject | Clathrin light chain; localizes to PM/TGN/cell plates and dynamic foci at the oil body membrane; EM shows clathrin-coated vesicles budding from it. |
| Mp6g08690 | MpERF13 | experimental subject | MpERF13 locus; master ERF/AP2 TF for oil body formation. GOF (~6x oil bodies), KO abolishes oil bodies. |
| Mp6g00050 | MpSYP12A | experimental subject | Syntaxin-1/SYP1 homolog (KNOLLE-like); localizes to forming cell plates; KO not recoverable (likely essential), mutant cells enlarged with wall stubs — role in cytokinesis. |
| Mp4g20670 | MpSYP12B | experimental subject | Oil-body-cell-specific SYP1; localizes to oil body membrane; its promoter redirects SNAREs/cargo to the oil body — central to the redirection model. |
| Mp7g13190 | MpSYP13A | experimental subject | PM SYP1 SNARE; dual oil body+PM localization, redirected to oil body under MpSYP12B promoter. |
| Mp2g19600 | MpSYP13B | experimental subject | PM SYP1 SNARE; PM-restricted normally, redirected to oil body membrane under MpSYP12B promoter. |
| Mp3g25140 | MpAPT | experimental tool | MpAPT locus; RT-qPCR reference. |
| — | MpCYCB1 missing | experimental tool | CYCB1 promoter used to drive cell-cycle-dependent expression. |
| Mp3g23400 | MpEF1 | experimental tool | Constitutive EF1alpha promoter used for constructs. |
| Mp1g19070 | MpGOS11 | experimental tool | Golgi SNARE marker; compartment control. |
| Mp4g17210 | MpPIP2 | experimental tool | PM aquaporin marker; redirected to oil body under MpSYP12B promoter. |
| Mp1g13670 | MpSEC20 | experimental tool | ER SNARE marker; compartment control. |
| Mp2g11050 | MpSEC22 | experimental tool | ER SNARE marker; compartment control. |
| Mp3g06390 | MpSFT1 | experimental tool | Golgi SNARE marker; compartment control. |
| Mp8g15260 | MpSYP2 | experimental tool | Tonoplast SNARE marker; used to exclude tonoplast identity. |
| Mp2g14140 | MpSYP4 | experimental tool | TGN SNARE marker; compartment control. |
| Mp3g18380 | MpSYP6A | experimental tool | TGN SNARE marker; compartment control. |
| Mp2g09390 | MpTUB2 | experimental tool | pENTR MpTUB2 entry clone used in cloning. |
| Mp1g18750 | MpUSE1A | experimental tool | ER SNARE marker; compartment control. |
| Mp8g00880 | MpVAMP71 | experimental tool | Tonoplast SNARE marker; compartment control. |
| Mp3g02320 | MpC1HDZ | citation background | TF cited from other work; mutant has fewer oil bodies. Not studied here. |
| Mp3g07510 | MpMYB02 | citation background | MpMYB02 locus; cited driver of marchantin A, expression positively regulated by MpERF13. |